6.4.1 Assumptions Underlying Parametric Tests
Normality: The data must be approximately normally distributed, formally assessed using the Shapiro-Wilk test for sample sizes below approximately 50,...
PharmacologyPhase 6 — Statistical Analysis & Data Interpretation6.4 Parametric Tests: The t-Test and ANOVA1 min readUpdated 2026-07-13
- Normality: The data must be approximately normally distributed, formally assessed using the Shapiro-Wilk test for sample sizes below approximately 50, or the Kolmogorov-Smirnov test for larger samples.
- Homogeneity of variance: The groups being compared should have approximately equal variance, assessed using Levene's test or the F-test for two groups, or Bartlett's test across multiple groups.
- Independence: Individual observations must be independent of one another, except in explicitly paired or repeated-measures designs.
- Scale of measurement: The outcome variable must be measured on an interval or ratio scale, not an ordinal or nominal scale.
- Violated assumptions: If any of the above assumptions is violated, the appropriate non-parametric equivalent should be used, or the data may be mathematically transformed (commonly by logarithmic or square-root transformation) before re-testing for normality.