Pharmacology
Phase 6 — Statistical Analysis & Data Interpretation
6.4 Parametric Tests: The t-Test and ANOVA
6.4.1 Assumptions Underlying Parametric Tests

6.4.1 Assumptions Underlying Parametric Tests

Normality: The data must be approximately normally distributed, formally assessed using the Shapiro-Wilk test for sample sizes below approximately 50,...

PharmacologyPhase 6 — Statistical Analysis & Data Interpretation6.4 Parametric Tests: The t-Test and ANOVA1 min readUpdated 2026-07-13
  • Normality: The data must be approximately normally distributed, formally assessed using the Shapiro-Wilk test for sample sizes below approximately 50, or the Kolmogorov-Smirnov test for larger samples.
  • Homogeneity of variance: The groups being compared should have approximately equal variance, assessed using Levene's test or the F-test for two groups, or Bartlett's test across multiple groups.
  • Independence: Individual observations must be independent of one another, except in explicitly paired or repeated-measures designs.
  • Scale of measurement: The outcome variable must be measured on an interval or ratio scale, not an ordinal or nominal scale.
  • Violated assumptions: If any of the above assumptions is violated, the appropriate non-parametric equivalent should be used, or the data may be mathematically transformed (commonly by logarithmic or square-root transformation) before re-testing for normality.

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