Phase 3 – Structural Characterization Techniques
Phase 3 – Structural Characterization Techniques contains 8 topic pages in Pharmaceutical Chemistry.
Learning Objectives
- Explain how UV-Visible and FTIR spectroscopy contribute preliminary structural information.
- Describe the principle of NMR spectroscopy and the complementary information provided by 1D and 2D experiments.
- Explain the principle of mass spectrometry and the additional structural confidence provided by HRMS.
- Describe LC-MS/MS and GC-MS as hyphenated techniques combining separation with mass detection.
- Explain how spectral data from multiple techniques is integrated to confirm molecular structure.
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Topics and Topic Groups
Browse the available learning units in this chapter.
UV-Visible (UV-Vis) Spectroscopy
Ultraviolet-visible spectroscopy measures the absorption of light in the 200–800 nanometre range arising from electronic transitions within a molecule's...
FTIR Spectroscopy
Fourier-transform infrared spectroscopy identifies the functional groups present within a molecule by their characteristic vibrational absorption...
Nuclear Magnetic Resonance (NMR) Spectroscopy
Introduction and Principle Nuclear magnetic resonance spectroscopy exploits the intrinsic magnetic property (spin) of certain atomic nuclei, most...
Mass Spectrometry
Mass spectrometry determines the molecular mass of a compound, and through analysis of its characteristic fragmentation pattern, provides additional...
HRMS (High-Resolution Mass Spectrometry)
High-resolution mass spectrometry measures mass-to-charge ratio with sufficient precision — typically to within a few parts per million — to distinguish...
LC–MS/MS
Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combines chromatographic separation with sequential stages of mass analysis, in which a...
GC–MS
Gas chromatography coupled to mass spectrometry (GC-MS) applies the same hyphenated separation-and-detection principle to volatile and thermally stable...
Structural Interpretation, Spectral Analysis, and Structure Confirmation
Definitive structure confirmation of a synthesised compound integrates evidence from every spectroscopic and spectrometric technique described in this phase...